Real-time PCR for sexing Schistosoma mansoni cercariae
نویسندگان
چکیده
منابع مشابه
PCR effectiveness for sexing Schistosoma mansoni cercariae: application for sexing clonal cercarial populations.
Schistosoma mansoni is a digenean parasite known for its importance as a human pathogen. Compared with the other genera belonging to the digenean families, the Schistosoma genus is characterized by having acquired gonochorism from hermaphroditic ancestors [1]. Sex is genetically determined in the egg [2] and female schistosome is heterogametic (ZW) such as lepidoptera, reptiles and birds. A mar...
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The present study describes a real-time PCR approach with high resolution melting-curve (HRM) assay developed for the detection and differentiation of Schistosoma mansoni and S. haematobium in fecal and urine samples collected from rural Yemen. The samples were screened by microscopy and PCR for the Schistosoma species infection. A pair of degenerate primers were designed targeting partial regi...
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BACKGROUND In Schistosoma mansoni infection, diagnosis and control after treatment mainly rely on parasitological stool investigations which are laborious and have limited sensitivity. PCR methods have shown equal or superior sensitivity but preservation and storage methods limit their use in the field. Therefore, the use of occult blood detection cards (fecal cards) for easy sampling and stora...
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The motility of many parasites is critical for the infection process of their host, as exemplified by the transmission cycle of the blood fluke Schistosoma mansoni [1]. In their human infectious stage, immature, submillimetre-scale forms of the parasite known as cercariae swim in freshwater and infect human hosts by penetrating through the skin [1, 2]. This infection causes Schistosomiasis, a p...
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ژورنال
عنوان ژورنال: Molecular and Biochemical Parasitology
سال: 2016
ISSN: 0166-6851
DOI: 10.1016/j.molbiopara.2016.03.010